Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using Biotin-65563

  1x10^6 human peripheral blood leukocytes were unstained or surface stained with 0.25 ug Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a RecAb (Biotin-65563, Clone: TS2/4) and CoraLite®488-conjugated streptavidin. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using Biotin-65563

  1x10^6 human peripheral blood leukocytes were unstained (blue) or surface stained with 5 ul Biotin Anti-Human CD11a (TS2/4) Mouse IgG2a RecAb (Biotin-65563, Clone: TS2/4) and CoraLite®488-conjugated streptavidin (red). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD11a Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65563

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Biotin

Unconjugated APC-Cyanine7 Biotin CoraLite® Plus 405 CoraLite® Plus 488 CoraLite® Plus 647 CoraLite® Plus 750 CoraLite®594 FITC Plus PE PE-Cyanine7 PerCP-Cyanine5.5

Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65563 targets CD11a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: HLA-DR CTL line
• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: CD11a
• Gene ID (NCBI): 3683
• ENSEMBL Gene ID: ENSG00000005844
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20701
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).