Atlantic Blue™ Anti-Human CD11a (TS2/4)

• 

  FC experiment of human PBMCs using AB-65194

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD11a (AB-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using AB-65194

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD11a (AB-65194, Clone:TS2/4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD11a Monoclonal Antibody for FC

Cat No. AB-65194

Clone No.TS2/4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65194 targets CD11a in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: HLA-DR CTL line
• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: CD11a
• Gene ID (NCBI): 3683
• ENSEMBL Gene ID: ENSG00000005844
• RRID: AB_2935190
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P20701
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).