Anti-Mouse IFNGR1/CD119 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98201-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD119 / IFN-gamma R1 Rabbit Recombinant Antibody (98201-1-RR, Clone: 241832H4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98201-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98201-1-RR against Mouse CD119 / IFN-gamma R1 were performed. The affinity constant is below 1 pM.

IFNGR1/CD119 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98201-1-RR

Clone No.241832H4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN-gamma R1, CD119

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98201-1-RR targets IFNGR1/CD119 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon gamma receptor 1
• Calculated Molecular Weight: 52kDa
• GenBank Accession Number: NM_010511.3
• Gene Symbol: Ifngr1
• Gene ID (NCBI): 15979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15261
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)