Anti-Mouse CD117/c-Kit Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using 98041-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD117/c-kit Rabbit Recombinant Antibody (98041-1-RR, Clone: 240246C11) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2) (CL647-65139, Clone: RA3-6B2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98041-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD117/c-kit Rabbit Recombinant Antibody (98041-1-RR, Clone: 240246C11)(red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98041-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98041-1-RR against Mouse CD117/c-Kit were performed. The affinity constant is 0.281 nM.

CD117/c-Kit Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98041-1-RR

Clone No.240246C11

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC

Synonyms

Kit, CD117, c KIT, 240246C11

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98041-1-RR targets CD117/c-Kit in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: kit oncogene
• Calculated Molecular Weight: 109 kDa
• GenBank Accession Number: NM_001122733.1
• Gene Symbol: CD117
• Gene ID (NCBI): 16590
• RRID: AB_3672188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05532-1
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).