Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody, PBS Only

CD107b / LAMP2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4B4

Cat no : 65520-1-PBS

Synonyms

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

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Validation Data Gallery

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  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.2 ug Anti-Human CD107b Mouse Monoclonal Antibody (Clone:H4B4) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65520-1-PBS targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human adherent peripheral blood cells
• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).