Anti-Human CD107a / LAMP1 (H4A3), PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

WB, IF/ICC, FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4A3

Cat no : 65051-1-PBS

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

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Validation Data Gallery

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  WB analysis using 65051-1-Ig (same clone as 65051-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 65051-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

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Product Information

65051-1-PBS targets CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human adherent peripheral blood cells
• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC006345
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• RRID: AB_2881467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).