CD100 Monoclonal antibody, PBS Only

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  WB analysis using 66582-1-Ig (same clone as 66582-1-PBS)

  various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66582-1-Ig (same clone as 66582-1-PBS)

  WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells. This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66582-1-Ig (same clone as 66582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66582-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CD100 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66582-1-PBS

Clone No.1C1A6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

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Product Information

66582-1-PBS targets CD100 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD100 fusion protein Ag26461
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC054500
• Gene Symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• RRID: AB_2881942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92854
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.