CD100 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD100 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C1A6

Cat no : 66582-1-Ig

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

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Validation Data Gallery

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  WB analysis using 66582-1-Ig

  various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66582-1-Ig

  WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells.

• 

  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, K-562 cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:225-1:900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66582-1-Ig targets CD100 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD100 fusion protein Ag26461
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC054500
• Gene Symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• RRID: AB_2881942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

Publications

Species	Application	Title
human	WB	Oncol Lett; Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer; Authors - Xiang Liu