CoraLite® Plus 488-conjugated CCT8 Monoclonal antibody

CCT8 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7C3

Cat no : CL488-67539

Synonyms

D21S246, Chaperonin containing T-complex polypeptide 1 subunit 8, CCT-theta, CCTQ, CCT theta

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-67539

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 CCT8 antibody (CL488-67539, Clone: 1A7C3 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL488-67539

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT8 (CL488-67539, Clone:1A7C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67539 targets CCT8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT8 fusion protein Ag30082
• Full Name: chaperonin containing TCP1, subunit 8 (theta)
• Calculated Molecular Weight: 548 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012584
• Gene Symbol: CCT8
• Gene ID (NCBI): 10694
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.