CCT8 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CCT8 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7C3

Cat no : 67539-1-PBS

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

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Validation Data Gallery

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  WB analysis using 67539-1-Ig (same clone as 67539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67539-1-Ig (same clone as 67539-1-PBS)

  WB result of CCT8 antibody (67539-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67539-1-Ig (same clone as 67539-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCT8 antibody (67539-1-Ig, Clone: 1A7C3 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67539-1-PBS targets CCT8 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT8 fusion protein Ag30082
• Full Name: chaperonin containing TCP1, subunit 8 (theta)
• Calculated Molecular Weight: 548 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC012584
• Gene Symbol: CCT8
• Gene ID (NCBI): 10694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.