CCT7 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CCT7 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1G1

Cat no : 67540-1-PBS

Synonyms

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

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Validation Data Gallery

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  WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

  WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67540-1-Ig (same clone as 67540-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67540-1-PBS targets CCT7 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT7 fusion protein Ag9502
• Full Name: chaperonin containing TCP1, subunit 7 (eta)
• Calculated Molecular Weight: 543 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC019296
• Gene Symbol: CCT7
• Gene ID (NCBI): 10574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.