TCP1 eta Monoclonal antibody

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• KD/KO Validated

TCP1 eta Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2C1G1

Cat no : 67540-1-Ig

Synonyms

CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH

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Validation Data Gallery

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  WB analysis using 67540-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 67540-1-Ig

  WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 67540-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67540-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 67540-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, HEK-293 cells, LNCaP cells, Hela cells, HepG cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67540-1-Ig targets TCP1 eta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TCP1 eta fusion protein Ag9502
• Full Name: chaperonin containing TCP1, subunit 7 (eta)
• Calculated Molecular Weight: 543 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC019296
• Gene Symbol: CCT7
• Gene ID (NCBI): 10574
• RRID: AB_2882759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita