CoraLite® Plus 488-conjugated CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F5A6

Cat no : CL488-60264

Synonyms

TCP 1 gamma, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma, CCTG

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-60264

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CCT3 antibody (CL488-60264, Clone: 5F5A6 ) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL488-60264

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT3 (CL488-60264, Clone:5F5A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60264 targets CCT3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCT3 fusion protein Ag0843
• Full Name: chaperonin containing TCP1, subunit 3 (gamma)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC006501
• Gene Symbol: CCT3
• Gene ID (NCBI): 7203
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.