CoraLite®594-conjugated CCR6 Monoclonal antibody

CCR6 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C11A9

Cat no : CL594-66801

Synonyms

BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22

---

Validation Data Gallery

• 

  IF Staining of human tonsillitis using CL594-66801

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CCR6 antibody (CL594-66801, Clone: 1C11A9 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66801

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CCR6 antibody (CL594-66801, Clone: 1C11A9 ) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66801 targets CCR6 in IF-P applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCR6 fusion protein Ag24195
• Full Name: chemokine (C-C motif) receptor 6
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC037960
• Gene Symbol: CCR6
• Gene ID (NCBI): 1235
• RRID: AB_2920041
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCR6, also known CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed on most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells and B-cells and plays a role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).