CCR2 Polyclonal antibody

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  WB analysis using 30420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30420-1-AP (CCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 30420-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2 antibody (30420-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CCR2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30420-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2, C C CKR 2

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Tested Applications

• Positive WB detected in: HaCaT cells, K-562 cells, THP-1 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30420-1-AP targets CCR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCR2 fusion protein Ag33071
• Full Name: chemokine (C-C motif) receptor 2
• Calculated Molecular Weight: 374 aa, 42 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC074751
• Gene Symbol: CCR2
• Gene ID (NCBI): 729230
• RRID: AB_3669721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 (C-C motif chemokine receptor-2), is a 42-kDa transmembrane protein highly expressed in bone marrow and lymph nodes (PMID: 16150057). It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1) (PMID: 8146186). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. (PMID: 19441905). This antibody recognizes a homodimer consisting of its isoform1 and isoform2 (70 kDa).