Anti-Mouse CCL3 Rabbit Recombinant Antibody

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  FC experiment of J774A.1 using 98194-1-RR

  1x10^6 LPS and Brefeldin A treated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone:241695B10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated J774A.1 cells were intracellularly stained with 0.25 ug Anti-Mouse CCL3 Rabbit Recombinant Antibody (98194-1-RR, Clone: 241695B10) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98194-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98194-1-RR against Mouse CCL3 were performed. The affinity constant is 3.87 nM.

CCL3 Recombinant Antibody for FC (Intra)

Cat No. 98194-1-RR

Clone No.241695B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Mip1a, Macrophage inflammatory protein 1-alpha, L2G25B, Heparin-binding chemotaxis protein, C-C motif chemokine 3

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated J774A.1 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98194-1-RR targets CCL3 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-C motif) ligand 3
• Calculated Molecular Weight: 10kd
• GenBank Accession Number: NM_011337
• Gene Symbol: Ccl3
• Gene ID (NCBI): 20302
• RRID: AB_3672331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10855
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.