CCDC94 Recombinant antibody, PBS Only (Capture)

CCDC94 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240517E9

Cat no : 83580-5-PBS

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517E9

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Validation Data Gallery

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  IHC staining of human intrahepatic cholangiocarcinoma using 83580-5-RR (same clone as 83580-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83580-5-RR (CCDC94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83580-5-RR (same clone as 83580-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-5-RR, Clone: 240517E9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83580-5-RR (same clone as 83580-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-5-RR, Clone:240517E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00553-1

  Cytometric bead array standard curve of MP00553-1, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-5-PBS. Detection antibody: 83580-4-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83580-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83580-5-RR against Human CCDC94 were performed. The affinity constant is 1.02 nM.

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Product Information

83580-5-PBS targets CCDC94 as part of a matched antibody pair:

MP00553-1: 83580-5-PBS capture and 83580-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCDC94 fusion protein Ag13764
• Full Name: coiled-coil domain containing 94
• Calculated Molecular Weight: 323 aa, 37 kDa
• GenBank Accession Number: BC000561
• Gene Symbol: CCDC94
• Gene ID (NCBI): 55702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)