CEP89, CCDC123 Polyclonal antibody

CEP89, CCDC123 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24002-1-AP

Synonyms

CEP89, CCDC123, Coiled-coil domain-containing protein 123, Cep123, Centrosomal protein of 89 kDa

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 24002-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 24002-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 24002-1-AP (CEP89 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, Neuro-2a cells
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 3 publications below

Product Information

24002-1-AP targets CEP89, CCDC123 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP89, CCDC123 fusion protein Ag21206
• Full Name: coiled-coil domain containing 123
• Calculated Molecular Weight: 783 aa, 90 kDa
• Observed Molecular Weight: 85-100 kDa, 40 kDa
• GenBank Accession Number: BC136328
• Gene Symbol: CEP89
• Gene ID (NCBI): 84902
• RRID: AB_2879400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	IF	PLoS Biol; The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly; Authors - Pierrick Le Borgne