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ACKR2 Monoclonal antibody

ACKR2 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A3A2

Cat no : 60046-1-Ig

Synonyms

ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ACKR2 Monoclonal antibody (60046-1-Ig)

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ACKR2 Monoclonal antibody (60046-1-Ig)

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ACKR2 Monoclonal antibody (60046-1-Ig)

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ACKR2 Monoclonal antibody (60046-1-Ig)

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60046-1-Ig targets ACKR2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACKR2 fusion protein Ag1941
Full Name chemokine binding protein 2
Calculated Molecular Weight 43 kDa
GenBank Accession NumberBC008816
Gene Symbol ACKR2
Gene ID (NCBI) 1238
RRIDAB_2071883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.

Protocols

Product Specific Protocols
IHC protocol for ACKR2 antibody 60046-1-IgDownload protocol
IF protocol for ACKR2 antibody 60046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols