ACKR2 Monoclonal antibody, PBS Only

ACKR2 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3A2

Cat no : 60046-1-PBS

Synonyms

ACKR2, C C chemokine receptor D6, CCBP2, CCR10, CCR9, chemokine binding protein 2, Chemokine binding protein D6, Chemokine receptor CCR 10, Chemokine receptor CCR 9, CMKBR9, D6, hD6

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Validation Data Gallery

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  IHC staining of human tonsillitis using 60046-1-Ig (same clone as 60046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60046-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60046-1-Ig (same clone as 60046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60046-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60046-1-Ig (same clone as 60046-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60046-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60046-1-Ig (same clone as 60046-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60046-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60046-1-PBS targets ACKR2 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACKR2 fusion protein Ag1941
• Full Name: chemokine binding protein 2
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC008816
• Gene Symbol: CCBP2
• Gene ID (NCBI): 1238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.