CCAR1 Recombinant antibody, PBS Only

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  WB analysis using 83936-4-RR (same clone as 83936-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83936-4-RR (CCAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83936-4-RR (same clone as 83936-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (83936-4-RR, Clone: 240980C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

CCAR1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83936-4-PBS

Clone No.240980C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DIS, Death inducer with SAP domain, CARP-1, CARP1, CARP 1

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Product Information

83936-4-PBS targets CCAR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCAR1 fusion protein Ag18786
• Full Name: cell division cycle and apoptosis regulator 1
• Calculated Molecular Weight: 1150 aa, 133 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC132725
• Gene Symbol: CCAR1
• Gene ID (NCBI): 55749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8IX12
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.