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  • Western Blot (WB) analysis of various lysates using CCAR1 Recombinant antibody (83936-4-RR)

    WB analysis using 83936-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83936-4-RR (CCAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCAR1 Recombinant antibody (83936-4-RR)

    IF Staining of A431 using 83936-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (83936-4-RR, Clone: 240980C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CCAR1 Recombinant antibody

CCAR1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83936-4-RR
Clone No.240980C12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DIS, Death inducer with SAP domain, CARP-1, CARP1, CARP 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83936-4-RR targets CCAR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CCAR1 fusion protein Ag18786 Predict reactive species
Full Name cell division cycle and apoptosis regulator 1
Calculated Molecular Weight 1150 aa, 133 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC132725
Gene Symbol CCAR1
Gene ID (NCBI) 55749
RRIDAB_3671518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IX12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

Protocols

Product Specific Protocols
WB protocol for CCAR1 antibody 83936-4-RRDownload protocol
IF protocol for CCAR1 antibody 83936-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols