CBP80 Recombinant antibody, PBS Only

CBP80 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-PBS

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1

---

Validation Data Gallery

• 

  WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83212-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

---

Product Information

83212-4-PBS targets CBP80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBP80 fusion protein Ag0496
• Full Name: nuclear cap binding protein subunit 1, 80kDa
• Calculated Molecular Weight: 92 kDa
• GenBank Accession Number: BC001450
• Gene Symbol: NCBP1
• Gene ID (NCBI): 4686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.