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CBP80 Recombinant antibody

CBP80 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-RR

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBP80 Recombinant antibody (83212-4-RR)

    WB analysis using 83212-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBP80 Recombinant antibody (83212-4-RR)

    IF Staining of HepG2 using 83212-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity experiments of 83212-4-RR

    Affinity experiments of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, A431 cells, NCI-H1299 cells, PC-12 cells, HEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83212-4-RR targets CBP80 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBP80 fusion protein Ag0496
Full Name nuclear cap binding protein subunit 1, 80kDa
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC001450
Gene Symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Protocols

Product Specific Protocols
WB protocol for CBP80 antibody 83212-4-RRDownload protocol
IF protocol for CBP80 antibody 83212-4-RRDownload protocol
FC protocol for CBP80 antibody 83212-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols