CBLB Monoclonal antibody, PBS Only

CBLB Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3A2

Cat no : 66353-1-PBS

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66353-1-Ig (same clone as 66353-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66353-1-PBS targets CBLB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: CBLB fusion protein Ag18757
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Calculated Molecular Weight: 982 aa, 109 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032851
• Gene Symbol: CBLB
• Gene ID (NCBI): 868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.