CBLB Monoclonal antibody

CBLB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D3A2

Cat no : 66353-1-Ig

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 66353-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 66353-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66353-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66353-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 66353-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 66353-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of RAW 264.7 using 66353-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, rat heart tissue
• Positive IHC detected in: human heart tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66353-1-Ig targets CBLB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: CBLB fusion protein Ag18757
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Calculated Molecular Weight: 982 aa, 109 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032851
• Gene Symbol: CBLB
• Gene ID (NCBI): 868
• RRID: AB_2881733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	J Exp Clin Cancer Res; GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway; Authors - Haijun Bao