RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 66353-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 66353-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of RAW 264.7 using 66353-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human heart tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
RAW 264.7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunohistochemistry (IHC)
IHC : 1:100-1:400
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 66353-1-Ig
rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 66353-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 66353-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 66353-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 66353-1-Ig
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).
IF/ICC Figures
IF Staining of RAW 264.7 using 66353-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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