CoraLite® Plus 488-conjugated CAPZA1 Monoclonal antibody

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  IF Staining of MCF-7 using CL488-66066

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL488-66066 (CAPZA1 antibody) at dilution of 1:100.

• Featured Product
• KD/KO Validated

CAPZA1 Monoclonal Antibody for IF/ICC

Cat No. CL488-66066

Clone No.10H1F9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66066 targets CAPZA1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPZA1 fusion protein Ag18841
• Full Name: capping protein (actin filament) muscle Z-line, alpha 1
• Calculated Molecular Weight: 286 aa, 33 kDa
• GenBank Accession Number: BC000144
• Gene Symbol: CAPZA1
• Gene ID (NCBI): 829
• RRID: AB_2883248
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P52907
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)