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CoraLite® Plus 488-conjugated CAPNS1 Monoclonal antibody

CAPNS1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B6G6

Cat no : CL488-67838

Synonyms

CAPN4, CAPNS, Calpain small subunit 1, Calpain regulatory subunit, Calcium-dependent protease small subunit 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated CAPNS1 Monoclonal an (CL488-67838)

    IF Staining of A431 using CL488-67838

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CAPNS1 antibody (CL488-67838, Clone: 2B6G6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CAPNS1 Monoclonal an (CL488-67838)

    FC experiment of HeLa using CL488-67838

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAPNS1 (CL488-67838, Clone:2B6G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67838 targets CAPNS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPNS1 fusion protein Ag21885
Full Name calpain, small subunit 1
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC011903
Gene Symbol CAPNS1
Gene ID (NCBI) 826
RRIDAB_2919558
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPNS1 is also named as CANP, CDPS, CSS1, CANPS, CAPN4 and CALPAIN4. It is a member of the calpain small subunit family, and belongs to the calcium-dependent family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. The small regulatory subunit consists of an N-terminal domain, containing about 30% glycine residues and a C-terminal Ca-binding domain (PMID: 9228946). This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CAPNS1 antibody CL488-67838Download protocol
Standard Protocols
Click here to view our Standard Protocols