CAP2 Monoclonal antibody, PBS Only

CAP2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7D2

Cat no : 67412-1-PBS

Synonyms

CAP 2, CAP2

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Validation Data Gallery

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  WB analysis using 67412-1-Ig (same clone as 67412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67412-1-Ig (same clone as 67412-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67412-1-Ig (same clone as 67412-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 67412-1-Ig (same clone as 67412-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67412-1-Ig (same clone as 67412-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67412-1-Ig (same clone as 67412-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67412-1-PBS in a different storage buffer formulation.

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Product Information

67412-1-PBS targets CAP2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAP2 fusion protein Ag8622
• Full Name: CAP, adenylate cyclase-associated protein, 2 (yeast)
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 51-53 kDa
• GenBank Accession Number: BC008481
• Gene Symbol: CAP2
• Gene ID (NCBI): 10486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.