CoraLite® Plus 488-conjugated CAP1 Monoclonal antibody

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  IF Staining of HepG2 using CL488-68207

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-68207

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

CAP1 Monoclonal Antibody for IF/ICC

Cat No. CL488-68207

Clone No.2H3A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68207 targets CAP1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAP1 fusion protein Ag8876
• Full Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Calculated Molecular Weight: 475 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC013963
• Gene Symbol: CAP1
• Gene ID (NCBI): 10487
• RRID: AB_3084441
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q01518
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.