CAMK4 Monoclonal antibody

CAMK4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7A7

Cat no : 68452-1-Ig

Synonyms

CaM kinase GR, CaMK GR, CaMK IV, CAMK4

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Validation Data Gallery

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  WB analysis using 68452-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68452-1-Ig (CAMK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68452-1-Ig targets CAMK4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAMK4 fusion protein Ag33332
• Full Name: calcium/calmodulin-dependent protein kinase IV
• Calculated Molecular Weight: 473 aa, 52 kDa
• Observed Molecular Weight: 56-65 kDa
• GenBank Accession Number: BC025687
• Gene Symbol: CAMK4
• Gene ID (NCBI): 814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)