CALD1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CALD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E1C11

Cat no : 66693-1-PBS

Synonyms

Caldesmon, CDM, caldesmon 1, CAD, 1E1C11

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Validation Data Gallery

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  WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

  WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of human hysteromyoma using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

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Product Information

66693-1-PBS targets CALD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CALD1 fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated Molecular Weight: 538 aa, 63 kDa
• Observed Molecular Weight: 70 kDa~75 kDa
• GenBank Accession Number: BC040354
• Gene Symbol: CALD1
• Gene ID (NCBI): 800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.