Carbonic Anhydrase IX/CA9 Recombinant antibody

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  IHC staining of mouse stomach using 84233-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 84233-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84233-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84233-1-RR against Human CA9 were performed. The affinity constant is below 1 pM.

Carbonic Anhydrase IX/CA9 Uni-rAb^TM Recombinant Antibody for IHC, ELISA

Cat No. 84233-1-RR

Clone No.241573A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

CAIX, CA9, carbonic anhydrase IX, CA-IX, CA IX

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Tested Applications

• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84233-1-RR targets Carbonic Anhydrase IX/CA9 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Carbonic Anhydrase IX/CA9 fusion protein Ag36941
• Full Name: carbonic anhydrase IX
• Calculated Molecular Weight: 459 aa, 50 kDa
• GenBank Accession Number: BC014950
• Gene Symbol: CA9
• Gene ID (NCBI): 768
• RRID: AB_3671786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. (PMID: 31819036).