CA9 Recombinant antibody, PBS Only (Capture)

CA9 Uni-rAb^TM Recombinant Antibody for IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241573A4

Cat no : 84233-1-PBS

Synonyms

CAIX, carbonic anhydrase IX, Carbonate dehydratase IX, CA-IX, CA IX

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Validation Data Gallery

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  IHC staining of mouse stomach using 84233-1-RR (same clone as 84233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84233-1-RR (same clone as 84233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84233-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01136-3

  Cytometric bead array standard curve of MP01136-3, CA9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84233-1-PBS. Detection antibody: 84233-6-PBS. Standard: Ag36941. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84233-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84233-1-RR against Human CA9 were performed. The affinity constant is below 1 pM.

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Product Information

84233-1-PBS targets CA9 as part of a matched antibody pair:

MP01136-3: 84233-1-PBS capture and 84233-6-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CA9 fusion protein Ag36941
• Full Name: carbonic anhydrase IX
• Calculated Molecular Weight: 459 aa, 50 kDa
• GenBank Accession Number: BC014950
• Gene Symbol: CA9
• Gene ID (NCBI): 768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. (PMID: 31819036).