CoraLite® Plus 488-conjugated CA11 Recombinant antibody

CA11 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230390F7

Cat no : CL488-83082-4

Synonyms

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

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Validation Data Gallery

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  IF Staining of U2OS using CL488-83082-4

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 CA11 antibody (CL488-83082-4, Clone: 230390F7 ) at dilution of 1:200.

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  FC experiment of A431 using CL488-83082-4

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CA11 Recombinant antibody (CL488-83082-4, Clone:230390F7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83082-4 targets CA11 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CA11 fusion protein Ag33373
• Full Name: carbonic anhydrase XI
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC002662
• Gene Symbol: CA11
• Gene ID (NCBI): 770
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).