C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2H6

Cat no : 66140-1-PBS

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6

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Validation Data Gallery

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  WB analysis of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66140-1-Ig (same clone as 66140-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  IP experiment of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

  IP result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66140-1-Ig (same clone as 66140-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (66140-1-Ig, Clone: 3D2H6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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Product Information

66140-1-PBS targets C9orf72 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C9orf72 fusion protein Ag21080
• Full Name: chromosome 9 open reading frame 72
• Calculated Molecular Weight: 481 aa, 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020851
• Gene Symbol: C9orf72
• Gene ID (NCBI): 203228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.