CoraLite® Plus 488-conjugated C3/C3b/C3c Monoclonal antibody
C3/C3b/C3c Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A6B3
Cat no : CL488-66157
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66157 targets C3/C3b/C3c in IF/ICC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | C3/C3b/C3c fusion protein Ag15955 |
Full Name | complement component 3 |
Calculated Molecular Weight | 1663 aa, 187 kDa |
Observed Molecular Weight | 115 kDa |
GenBank Accession Number | BC150179 |
Gene Symbol | C3 |
Gene ID (NCBI) | 718 |
RRID | AB_2883273 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 C3/C3b/C3c antibody CL488-66157 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |