CoraLite® Plus 488-conjugated C3/C3b/C3c Polyclonal antibody

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  IF Staining of HepG2 using CL488-21337

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 C3/C3b/C3c antibody (CL488-21337) at dilution of 1:200.

• Featured Product
• KD/KO Validated

C3/C3b/C3c Polyclonal Antibody for IF/ICC

Cat No. CL488-21337

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21337 targets C3/C3b/C3c in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C3/C3b/C3c fusion protein Ag15537
• Full Name: complement component 3
• Calculated Molecular Weight: 1663 aa, 187 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC150179
• Gene Symbol: C3
• Gene ID (NCBI): 718
• RRID: AB_3084052
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01024
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in activating the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg, and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.