C20orf26 Polyclonal antibody

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  IHC staining of mouse liver using 31427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31427-1-AP (C20orf26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 31427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31427-1-AP (C20orf26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 31427-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C20orf26 antibody (31427-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

C20orf26 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 31427-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

CFAP61, SPGF84, CaM-IP3

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31427-1-AP targets C20orf26 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C20orf26 fusion protein Ag35425
• Full Name: chromosome 20 open reading frame 26
• GenBank Accession Number: BC031674
• Gene Symbol: C20orf26
• Gene ID (NCBI): 26074
• RRID: AB_3669978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8NHU2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C20orf26, also known as CFAP61 (cilia and flagella associated protein 61), is a protein-coding gene located on human chromosome 20. The protein is predicted to be involved in cilium movement and cilium organization, and it is likely to be located in the axoneme and motile cilium. CFAP61 is also predicted to colocalize with the radial spoke stalk, which is a part of the structural framework of cilia and flagella.