CoraLite® Plus 488-conjugated C1qC Monoclonal antibody

C1qC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5B4

Cat no : CL488-66268

Synonyms

C1Q C, C1QC, C1QG, FLJ27103

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Validation Data Gallery

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  IF Staining of HUVEC using CL488-66268

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 C1qC antibody (CL488-66268, Clone: 1C5B4 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66268 targets C1qC in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: C1qC fusion protein Ag10233
• Full Name: complement component 1, q subcomponent, C chain
• Calculated Molecular Weight: 245 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC009016
• Gene Symbol: C1QC
• Gene ID (NCBI): 714
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.