C1QA Monoclonal antibody, PBS Only

C1QA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10E3

Cat no : 67063-1-PBS

Synonyms

C1QA

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Validation Data Gallery

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  WB analysis of human plasma using 67063-1-Ig (same clone as 67063-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 67063-1-Ig (same clone as 67063-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67063-1-Ig (same clone as 67063-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (67063-1-Ig, Clone: 1B10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67063-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67063-1-PBS targets C1QA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: C1QA fusion protein Ag28622
• Full Name: complement component 1, q subcomponent, A chain
• Calculated Molecular Weight: 245 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC030153
• Gene Symbol: C1QA
• Gene ID (NCBI): 712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.