BrdU Monoclonal antibody, PBS Only

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  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IF Staining of L-929 using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66241-1-Ig (same clone as 66241-1-PBS)

  Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66241-1-PBS in a different storage buffer formulation.

BrdU Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 66241-1-PBS

Clone No.1B10E12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

BUdR, Bromodeoxyuridine, BrdUrd, 5-bromo-2-deoxyuridine

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Product Information

66241-1-PBS targets BrdU in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Compound
• Full Name: BrdU
• RRID: AB_2881630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf