Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 80713-1-RR
WB result of Beta Tubulin antibody (80713-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Tubulin transfected HEK-293 cells.
WB result of Beta Tubulin antibody (80713-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Tubulin transfected HEK-293 cells.
WB analysis of zebrafish using 80713-1-RR
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 80713-1-RR
IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug.
IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug.
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of HeLa using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 80713-1-RR
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 80713-1-RR
Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.
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HeLa cells, HEK-293 cells, zebrafish tissue, Jurkat cells, K-562 cells, Hsc-T6 cells, NIH/3T3 cells, mouse brain, rat brain
Positive IP detected in
HEK-293 cells
Positive IHC detected in
human colon tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse eye tissue
Positive IF/ICC detected in
C2C12 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
80713-1-RR targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 80713-1-RR
WB result of Beta Tubulin antibody (80713-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Tubulin transfected HEK-293 cells.
WB analysis of zebrafish using 80713-1-RR
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 80713-1-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 80713-1-RR
IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug.
IF-P Figures
IF Staining of mouse eye using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of C2C12 using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of HeLa using 80713-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of HEK-293 using 80713-1-RR
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 80713-1-RR
Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
Beta Tubulin Recombinant antibody
Catalog Number
80713-1-RR
Citations
5
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF-P : 1:50-1:500 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat, zebrafish
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
