Beta Tubulin Recombinant antibody, PBS Only

Beta Tubulin Recombinant Antibody for WB, IP, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

WB, IP, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O13

Cat no : 80713-1-PBS

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3

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Validation Data Gallery

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  WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80713-1-RR (same clone as 80713-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

  IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:66240-1-Ig 1:10000) with HEK-293 cells lysate 1360 ug. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human Beta Tubulin (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80713-1-PBS targets Beta Tubulin in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

• Tested Reactivity: Human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Tubulin fusion protein Ag0117
• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000748
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.