Beta Tubulin Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

Beta Tubulin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O13

Cat no : 80713-1-PBS

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

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Validation Data Gallery

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  WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

  WB result of Beta Tubulin antibody (80713-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  WB analysis of zebrafish using 80713-1-RR (same clone as 80713-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

  IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80713-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.

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Product Information

80713-1-PBS targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Tubulin fusion protein Ag0117
• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000748
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.