• Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-17565

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inMCF-7 cells, T-47D cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17565 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Catenin fusion protein Ag11763 Predict reactive species
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2919153
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Beta Catenin antibody CL488-17565Download protocol
FC protocol for CL Plus 488 Beta Catenin antibody CL488-17565Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...