Beta Arrestin 2 Recombinant antibody

Beta Arrestin 2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241470F8

Cat no : 82780-6-RR

Synonyms

ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2

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Validation Data Gallery

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  WB analysis using 82780-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82780-6-RR (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82780-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82780-6-RR against Human Beta Arrestin 2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82780-6-RR is Beta Arrestin 2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Arrestin 2 fusion protein Ag0227
• Full Name: arrestin, beta 2
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC007427
• Gene Symbol: Beta Arrestin 2
• Gene ID (NCBI): 409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.