Anti-Human Beta-2-Microglobulin Rabbit Recombinant Antibody

Beta-2-Microglobulin Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241308D5

Cat no : 98165-1-RR

Synonyms

B2M, 241308D5, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98165-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human B2M Rabbit Recombinant Antibody (98165-1-RR, Clone:241308D5) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98165-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98165-1-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 2.13 nM.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98165-1-RR is Beta-2-Microglobulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.