Beta-2-Microglobulin Monoclonal antibody, PBS Only

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  IF Staining of NCCIT using 68395-1-Ig (same clone as 68395-1-PBS)

  Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68395-1-Ig (same clone as 68395-1-PBS)

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 68395-1-Ig (same clone as 68395-1-PBS)

  1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed. This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA

Cat No. 68395-1-PBS

Clone No.1H3F4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Synonyms

B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

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Product Information

68395-1-PBS targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61769
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.