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CoraLite®555-conjugated Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B5B9

Cat no : CL555-66799

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799)

    IF Staining of NIH/3T3 using CL555-66799

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 Bcl2 antibody (CL555-66799, Clone: 1B5B9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799)

    FC experiment of NIH/3T3 using CL555-66799

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799, Clone:1B5B9)(red), or 0.5 ug CoraLite® Plus 555 Mouse IgG1 Isotype Control (1F8D3) (CL555-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66799 targets Bcl2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24261
Full Name B-cell leukemia/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_2919696
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
IF protocol for CL555 Bcl2 antibody CL555-66799Download protocol
Standard Protocols
Click here to view our Standard Protocols