CoraLite® Plus 488-conjugated Bcl2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Bcl2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3F7

Cat no : CL488-68103

Synonyms

Bcl2, Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL488-68103

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl2 antibody (CL488-68103, Clone: 1B3F7 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68103 targets Bcl2 in IF/ICC applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24261
• Full Name: B-cell leukemia/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM-009741
• Gene Symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.